MAD3 (MXD3) (C-term) Goat Polyclonal Antibody

CAT#: AP10055PU-N

MAD3 (MXD3) (C-term) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: Peptide ELISA: 1/4000 (Detection Limit).
  Western blot: No signal obtained yet but low background observed in NCI-H460, HepG2, Human Liver, Human Heart and Human Brain lysates at up to 3 µg/ml.
  Immunocytochemistry: This product has been successfully used by an anonymous Customer on Methanol-fixed and 0.5% TX100-permeabilized HeLa cells, staning the cytoplasm.
  Immunohistochemistry: An anonymous Customer found positive results on Human HeLa cells.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-Terminus of Human MAD3 (NP_112590.1)
• Specificity:
  This antibody is expected to recognise isoform a (NP_112590.1) only.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: MAX dimerization protein 3
• Database Link:
  Entrez Gene 83463 Human
  UniProt ID Q9BW11
• Background: MAD3 contains 1 basic helix-loop-helix (bHLH) domain. It is a transcriptional repressor and binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
• Synonyms: MAD-3, MXD3,Max-interacting transcriptional repressor MAD3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors