TrkB (NTRK2) pTyr515 Rabbit Polyclonal Antibody

CAT#: AP09481PU-S

TrkB (NTRK2) pTyr515 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using TRkB pTyr515 Antibody (Red).

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Immunofluorescence: 1/100~1/200.
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human TrkB around the phosphorylation site of Tyrosine 515 (P-Q-Y-pF-G).
• Specificity: Antibody AP09481PU detects endogenous levels of TrkB only when phosphorylated at Tyrosine 515.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: neurotrophic receptor tyrosine kinase 2
• Database Link:
  Entrez Gene 25054 RatEntrez Gene 4915 Human
  UniProt ID Q16620
• Background: Neurotrophins activate tyrosine kinase receptors of the Trk family (TrkA, TrkB and TrkC) and they all share a common low affinity receptor (p75NTR or p75). Although p75NTR does not appear to directly transduce NGF signals, this receptor appears to increase the responsiveness of the trk receptors and plays a role in the retrograde transport of neurotrophin signals from axon terminals to the cell body. The expression of trk gene neurotrophin receptors in mouse is tabulated by Barbacid (1994). NGF binds with high affinity and activates the TrkA tyrosine kinase receptor, which is apparently responsible for signal transduction. BDNF activates the TrkB tyrosine kinase receptor, which it shares with NT-4. NT-3 activates the TrkC tyrosine kinase receptor, but can also activate TrkA and TrkB receptors in certain cell systems. NT-4 primarily activates the TrkB tyrosine kinase receptor, which it shares with BDNF.
• Synonyms: TRKB, NTRK2
• Note: Molecular Weight: 140 kDa

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway