alpha 1 Catenin (CTNNA1) pSer641 Rabbit Polyclonal Antibody

CAT#: AP09467PU-N

alpha 1 Catenin (CTNNA1) pSer641 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from Hela cells untreated or treated with starvation using Alpha-Catenin pSer641 antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1/500~1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around the phosphorylation site of Serine 641 (D-D-Sp-D-F) derived from Human Alpha-Catenin.
• Specificity: This antibody detects endogenous levels of Alpha-Catenin only when phosphorylated at Serine 641.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: catenin alpha 1
• Database Link:
  Entrez Gene 12385 MouseEntrez Gene 1495 Human
  UniProt ID P35221
• Background: The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.
• Synonyms: Alpha E-catenin, Alpha Catenin
• Note: Molecular Weight: 100 kDa

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction