PLCG 2 (PLCG2) pTyr1217 Rabbit Polyclonal Antibody

CAT#: AP08037PU-S

PLCG 2 (PLCG2) pTyr1217 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-gamma 2 (phospho-Tyr1217) Antibody.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry (1/50-1/100).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human PLC-gamma 2 around the phosphorylation site of Tyrosine 1217 (F-L-Yp-D-T).
• Specificity: This antibody detects endogenous levels of PLC-gamma 2 only when phosphorylated at Tyrosine 1217.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: phospholipase C gamma 2
• Database Link:
  Entrez Gene 5336 Human
  UniProt ID P16885
• Background: Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases. The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.
• Synonyms: Phospholipase C-gamma-2, PLC-gamma-2, Phospholipase C-IV, PLC-IV