SMAD3 pSer425 Rabbit Polyclonal Antibody

CAT#: AP08027PU-N

SMAD3 pSer425 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SMAD3 (Phospho-Ser425) Antibody (Left) or the same peincubated with blocking peptide (Right).

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide Sequence around the phosphorylation site of Ser425 (C-S-S-V-pS) derived from Human SMAD3
• Specificity: This antibody detects endogenous levels of Smad3 only when phosphorylated at Serine 425.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: SMAD family member 3
• Database Link:
  Entrez Gene 4088 Human
  UniProt ID P84022
• Background: Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
• Synonyms: SMAD family member 3, SMAD 3, SMAD-3, Mad3, MAD-3, MADH3, MAD homolog 3, JV15-2
• Note: Molecular Weight: 52 kDa