HMG1 (HMGB1) Rabbit Polyclonal Antibody

CAT#: AP06659PU-N

HMG1 (HMGB1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of HMGB1 antibody in extracts from Jurkat cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 131-180 of Human HMGB1.
• Specificity: This antibody detects endogenous levels of HMG-1 protein.
  (region surrounding Ala164)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~25 kDa
• Gene Name: high mobility group box 1
• Database Link:
  Entrez Gene 3146 Human
  UniProt ID P09429
• Background: High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Research suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture, including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, ATF-2, c-Jun and members of the NFκB family to activate transcription. Additional studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 each contain two DNA-binding domains, termed HMG boxes. HMG proteins bind singlestranded DNA but induce conformational changes in double-stranded DNA alone.
• Synonyms: High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1, Amphoterin