NOS1 Rabbit Polyclonal Antibody

CAT#: AP06622PU-N

NOS1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NOS1 antibody in extracts from A549 cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 815-862 of Human NOS1.
• Specificity: This antibody detects endogenous levels of NOS1 protein.
  (region surrounding Tyr846)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 43,125,149,160 kDa
• Gene Name: nitric oxide synthase 1
• Database Link:
  Entrez Gene 4842 Human
  UniProt ID P29475
• Background: NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
• Synonyms: bNOS, nNOS, NOS type I, Neuronal NOS, Constitutive NOS, NC-NOS