PPAR gamma (PPARG) Rabbit Polyclonal Antibody

CAT#: AP06573PU-N

PPAR gamma (PPARG) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PPAR-? antibody in extracts from K562 cells.

• 

  Immunohistochemistry (IHC) analyzes of PPAR-? antibody in paraffin-embedded human placenta tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 90-120 of Human PPAR-γ.
• Specificity: This antibody detects endogenous levels of PPAR-gamma protein.
  (region surrounding Ile106)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~55 kDa
• Gene Name: peroxisome proliferator activated receptor gamma
• Database Link:
  Entrez Gene 5468 Human
  UniProt ID P37231
• Background: PPAR gamma is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPAR gamma activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPAR gamma may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPAR gamma2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPAR gamma2 gene maybe correlated with abdominal obesity in type 2 diabetes.
• Synonyms: PPARG, NR1C3