HDAC5 Rabbit Polyclonal Antibody

CAT#: AP06393PU-N

HDAC5 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of HDAC5 antibody in extracts from HepG2 cells.

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  Immunohistochemistry (IHC) analyzes of HDAC5 antibody in paraffin-embedded human breast carcinoma prostate carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 220-269 of Human HDAC5.
• Specificity: This antibody detects endogenous levels of HDAC5 protein.
  (region surrounding Pro253)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 121 kDa
• Gene Name: histone deacetylase 5
• Database Link:
  Entrez Gene 10014 Human
  UniProt ID Q9UQL6
• Background: HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
• Synonyms: Histone deacetylase 5, HD5, KIAA0600, Antigen NY-CO-9