p53 (TP53) Rabbit Polyclonal Antibody

CAT#: AP06270PU-N

p53 (TP53) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p53 antibody in extracts from COS7 treated with UV.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human p53.
• Specificity: This antibody detects endogenous levels of p53 protein.
  (region surrounding Glu3)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~53,43 kDa
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active (DNA-binding) conformations and is differentially activated through posttranslational modifications including phosphorylation and acetylation.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13