p21 (CDKN1A) Rabbit Polyclonal Antibody

CAT#: AP06263PU-N

p21 (CDKN1A) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p21 Cip1 Antibody in extracts from Hela cell treated with EGF 200ng/ml 30'

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  Immunohistochemistry (IHC) analyzes with p21 Cip1 antibody on paraffin-embedded sections

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human P21.
• Specificity: This antibody detects endogenous levels of p21 Cip1 protein.
  (region surrounding Gly139)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~21.0 kDa
• Gene Name: cyclin-dependent kinase inhibitor 1A
• Database Link:
  Entrez Gene 1026 Human
  UniProt ID P38936
• Background: It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20.
• Synonyms: CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1