MMP13 Rabbit Polyclonal Antibody

CAT#: AP06226PU-N

MMP13 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MMP-13 antibody at 1/500 dilution Lane 1:Hela whole cell lysate treated with IL-1β Lane 2:sp2/0 whole cell lysate treated with IL-1β Lane 3:H9C2 whole cell lysate treated with IL-1β

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  Immunohistochemistry (IHC) analyzes of MMP-13 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human MMP-13.
• Specificity: This antibody detects endogenous levels of MMP-13 protein.
  (region surrounding Leu22)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 48, 60 kDa
• Gene Name: matrix metallopeptidase 13
• Database Link:
  Entrez Gene 4322 Human
  UniProt ID P45452
• Background: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.
• Synonyms: Collagenase 3, MMP13, Matrix metalloproteinase-13