Cytokeratin 8 (KRT8) Rabbit Polyclonal Antibody

CAT#: AP06205PU-N

Cytokeratin 8 (KRT8) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Cytokeratin 8 antibody at 1/500 dilution Lane 1: Hela whole cell lysate Lane 2: NIH-3T3 whole cell lysate Lane 3: H9C2 whole cell lysate

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  Immunohistochemistry (IHC) analyzes of Keratin 8 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 431-480 of Human Cytokeratin 8.
• Specificity: This Antibody detects endogenous levels of Keratin 8 protein.
  (region surrounding Val463)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 55 kDa
• Gene Name: keratin 8
• Database Link:
  Entrez Gene 3856 Human
  UniProt ID P05787
• Background: Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
• Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A