MEK5 (MAP2K5) pSer311/pThr315 Rabbit Polyclonal Antibody

CAT#: AP05673PU-N

MEK5 (MAP2K5) pSer311/pThr315 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:1000; detects a band of approximately 49kDa in rat testes cell lysates.
• Reactivities: Bovine, Canine, Chicken, Human, Monkey, Mouse, Rat, Xenopus, Zebrafish
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes phoshorylated Ser311 and Thr315.
• Specificity: This antibody specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315.
  Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.
• Formulation: 10mM Hepes pH7.5 containing 0.09% Sodium Azide (NaN3), 0.01% Bovine Serum Albumin, 50% Glycerol
  State: Purified
  State: Liquid purified IgG
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase kinase 5
• Database Link:
  Entrez Gene 5607 Human
  UniProt ID Q13163
• Background: MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation.
• Synonyms: MAP kinase kinase 5, MAPKK 5, MEK5, MKK5