Sphingomyelin Synthase 2 (SGMS2) Rabbit Polyclonal Antibody

CAT#: AP05278PU-N

Sphingomyelin Synthase 2 (SGMS2) rabbit polyclonal antibody, Aff - Purified

Product Images

SGMS2 antibody staining of Human brain lysate (10 ug/lane) at 0.5 ug/ml. Blot was developed with Pierce's Super Sytem West Femto - 1 minute exposure. Lane A: Antibody (1 ug/ml) without blocking peptide. Lane B: Antibody with 3 ug blocking peptide.

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  SGMS2 antibody staining of Human kidney section at 2 ug/ml. Anti-rabbit Ig (H+L) HRP conjugate (5 ug/ml) without antigen retrieval.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/400 as starting dilution.
  Immunohistochemistry: 1/400 as starting dilution.
  Positive Control: Brain, heart, kidney, liver, muscle and stomach.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from the Human Sphingomyelin Synthase 2 protein
• Specificity: This antibody detects Sphingomyelin Synthase 2.
• Formulation: PBS, pH 7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Antigen Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: sphingomyelin synthase 2
• Database Link:
  Entrez Gene 166929 Human
  UniProt ID Q8NHU3
• Background: Sphingomyelin (SM) is a major component of animal plasma membranes. Its production involves the transfer of phosphocholine from phosphatidylcholine and then onto ceramide. This also yields diacylglycerol as a side product. The reaction is catalysed by SM synthase, an important enzyme in the regulation of diacylglycerol and ceramide as anti- and proapoptotic stimuli. SM synthesis occurs in the lumen of the Golgi as well as on or at the cell surface. It is uncertain which SM enzyme forms are present at these differnet cellular locations. Human, mouse and Caenorhabditis elegans genomes each contain at least two different SM synthase (SMS) genes. Human SMS1 is localised to the Golgi, SMS2 resides primarily at the plasma membrane.
• Synonyms: Sphingomyelin synthase 2, SMS2
• Note: Predicted Molecular Weight: 42 kDa

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism