Her2 (ERBB2) Rabbit Polyclonal Antibody

CAT#: AP02612PU-S

Her2 (ERBB2) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 3. Western blot analysis of extracts from MCF and SKOV3 cells using HER2 antibody.

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  Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HER2 antibody (Left) and the same antibody preincubated with blocking peptide (Right).

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  Figure 3. Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around amino acids 1246~1250 (P-E-Y-L-G) derived from Human HER2.
• Specificity: This antibody detects endogenous levels of total HER2 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  Entrez Gene 2064 Human
  UniProt ID P04626
• Background: ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
• Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
• Note: Molecular Weight: 185 kDa