MITF pSer180 Rabbit Polyclonal Antibody

CAT#: AP01837PU-N

MITF pSer180 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MITF of p-MITF Antibody at 1/500 dilution. Lane 1: HEK293T cell lysate. Lane 2: Raw264.7 cell lysate. Lane 3: PC12 cell lysate.

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  Immunohistochemistry (IHC) analyzes of p-MITF Antibody (pSer180) in paraffin-embedded human breast carcinoma tissue at 1/100.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human MITF around the phosphorylation site of Serine 180.
• Specificity: This antibody detects endogenous levels of MITF protein only when phosphorylated at Serine 180.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 59 kDa
• Gene Name: melanogenesis associated transcription factor
• Database Link:
  Entrez Gene 4286 Human
  UniProt ID O75030
• Background: MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.
• Synonyms: Microphthalmia-associated transcription factor, Mi-protein