VEGF Receptor 2 (KDR) pTyr1175 Rabbit Polyclonal Antibody

CAT#: AP01716PU-N

VEGF Receptor 2 (KDR) pTyr1175 rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry analysisis of p-Flk-1 antibody (Cat.-No AP01716PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody reacts to CD309 / VEGFR-2 / Flk-1 only when phosphorylated at Tyr1175.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 230 kDa
• Gene Name: kinase insert domain receptor
• Database Link:
  Entrez Gene 3791 Human
  UniProt ID P35968
• Background: Epidermal growth factor mediates its effects on cell growth through its interaction with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGFalpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy-terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173 are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptor.
• Synonyms: VEGFR2, FLK1, KDR, VEGF Receptor 2