p53 (TP53) pSer15 Rabbit Polyclonal Antibody

CAT#: AP01656PU-N

p53 (TP53) pSer15 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p53 pSer15 antibody (Cat.-No.: AP01656PU-N) in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analysis of p53 pSer15 antibody (Cat.-No.: AP01656PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunoprecipitation: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p53 pSer15 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 43 kDa
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: p53 is a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor that up-regulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is up-regulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through post-translational modifications including phosphorylationand acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with CIS elements and are implicated in several human cancers. Phosphorylation of p53 at residue Thr 155 is mediated by the COP9 signalosome (CSN) and targets p53 to ubiquitin-26S proteasome-dependent degradation.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13