IGFBP5 Rabbit Polyclonal Antibody

CAT#: AP01139PU-S

IGFBP5 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot (Unreduced) using IGFBP5 Antibody Cat.-No AP01139PU

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  Western Blot (Reduced) using IGFBP5 Antibody Cat.-No AP01139PU

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  Sandwich ELISA using IGFBP5 Antibody Cat.-No AP01139PU

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  This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Indirect ELISA: To detect hIGF-BP5 (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5.
  Sandwich ELISA: To detect hIGF-BP5 (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human IGF-BP5 (Cat.-No AP01139BT-N or AP01139BT-S) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5.
  Western blot: To detect hIGF-BP5 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunofluorescence: This antibody stained U-2 OS and U-251 MG cells.
  The primary antibody was incubated at 2.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived recombinant Human IGF-BP5 (Cat.-No PA522)
• Specificity: This antibody detects Insulin-like Growth Factor Binding Protein 5.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: insulin like growth factor binding protein 5
• Database Link:
  Entrez Gene 3488 Human
  UniProt ID P24593
• Background: IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.
• Synonyms: IGF-binding protein 5, IGFBP-5, IBP5, IBP-5