SIGLEC10 Mouse Monoclonal Antibody [Clone ID: 5G6]

CAT#: AM60044FC-N

SIGLEC10 mouse monoclonal antibody, clone 5G6, FITC, Purified

Product Images

Human peripheral blood lymphocytes stained with mouse anti-human siglec-10-FITC clone 5G6.

Specifications

Product Data

• Clone Name: 5G6
• Applications: FC
• Recommended Dilution: Flow cytometry: Neat - 1/10, use 10ul of the suggested working dilution to label 10e6 cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human SIGLEC10, fused with the Fc region of human IgG
• Specificity: This antibody clone 5G6 specifically recognizes human SIGLEC10.
  Studies have shown that clone 5G6 does not cross react with SIGLECs 3, 5, 7, 8 and 9 (Munday J. et al.)
• Formulation: PBS containing 0.09% Sodium azide and 1% BSA
  Label: FITC
  State: Purified
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: sialic acid binding Ig like lectin 10
• Database Link:
  Entrez Gene 89790 Human
  UniProt ID Q96LC7
• Background: Siglec10 (Sialic acid binding Ig like lectin 10), a putative adhesion molecule and member of the Ig superfamily, is expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16⁺CD56^- natural killer (NK) cells.
  Structurally, Siglec10 is most similar to the CD33 related group of Siglecs, and preferentially binds to glycoconjugates containing alpha 2,3 or alpha2,6 linked sialic acid. Studies have shown that Siglec10 acts as a substrate for VAP1 (Vascular adhesion protein1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking (Kive, E. et al.). This interaction between Siglec10 and VAP1, implicates Siglec10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment.
• Synonyms: Siglec-10, SLG2