TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: TG86+P/T1]
CAT#: AM50312PU-T
TGF alpha (TGFA) mouse monoclonal antibody, clone TG86+P/T1, Purified
Size: 100 ug
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Specifications
Product Data | |
Clone Name | TG86+P/T1 |
Applications | FC, IF, IP, WB |
Recommended Dilution | Western Blotting (Not tested). Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 1-2 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 1-2 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer. |
Reactivities | Human, Rabbit, Zebrafish |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | A 10-amino acid synthetic peptide (aa34-43) from human TGFα (TG86 and P/T1). |
Specificity | This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this Monoclonal Antibody is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Cellular Localization: Cytoplasmic and secreted. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | ~6 kDa |
Gene Name | transforming growth factor alpha |
Database Link | |
Synonyms | Transforming growth factor alpha, ETGF |
Reference Data |
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