TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: P/T1]

CAT#: AM50311PU-S

TGF alpha (TGFA) mouse monoclonal antibody, clone P/T1, Purified

Specifications

Product Data

• Clone Name: P/T1
• Applications: FC, IF, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Western Blotting (Not tested).
  Immunofluorescence: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 1-2 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer.
• Reactivities: Human, Rabbit, Zebrafish
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A 10-amino acid synthetic peptide (aa34-43) from human TGFα.
• Specificity: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this Monoclonal Antibody is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
  Cellular Localization: Cytoplasmic and secreted.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~6 kDa
• Gene Name: transforming growth factor alpha
• Database Link:
  Entrez Gene 7039 Human
  UniProt ID P01135
• Synonyms: Transforming growth factor alpha, ETGF