IGF1 Mouse Monoclonal Antibody [Clone ID: SPM406]

CAT#: AM50117PU-T

IGF1 mouse monoclonal antibody, clone SPM406, Purified

Specifications

Product Data

• Clone Name: SPM406
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  In-vitro Neutralization of Biological Activity of IGF-1 (Use Azide Free Antibody).
  Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein.
• Reactivities: Human, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human IGF-1 protein.
• Specificity: This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin.
  Cellular Localization: Cytoplasmic (Secreted).
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~7.6 kDa
• Gene Name: insulin like growth factor 1
• Database Link:
  Entrez Gene 3479 Human
  UniProt ID P05019
• Background: IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon
• Synonyms: IGF-I, Somatomedin-C, Mechano growth factor, MGF, IBP1