EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: VU-3D1]

CAT#: AM32168PU-N

EMA (MUC1) mouse monoclonal antibody, clone VU-3D1, Purified

Specifications

Product Data

• Clone Name: VU-3D1
• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on frozen sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A Balb/c mouse was immunized with live cells (10e7 cells/dose in PBS) of human breast cancer cell line ZR-75-1. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by subcloning four times.
• Specificity: VU-3D1 reacts with MUC1.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: mucin 1, cell surface associated
• Database Link:
  Entrez Gene 4582 Human
  UniProt ID P15941
• Background:

  MUC1 is a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of mAb VU3D1 is the 8-mer SAPDTRPA of the MUC1 tandem repeat as established with “epitope fingerprinting”.

• Synonyms: MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3