6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: 10A12]

CAT#: AM26391PU-N

6xHistidine Epitope Tag (HHHHHH) mouse monoclonal antibody, clone 10A12, Purified

Product Images

Western blotting using anti-His antibodies (HisH8 and 10A12) to detect His-tagged HIV p24

Specifications

Product Data

• Clone Name: 10A12
• Applications: WB
• Recommended Dilution: Western blot.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic 6 x Histidine peptide conjugated with KLH
• Specificity: This antibody binds to His-tagged recombinant protein, but not with KLH.
• Formulation: 0.01M PBS pH 7.2
  State: Purified
  State: Lyophilized purified IgG fraction
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.