6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: 16-001]

CAT#: AM26097PU-N

6xHistidine Epitope Tag (HHHHHH) mouse monoclonal antibody, clone 16-001, Aff - Purified

Specifications

Product Data

• Clone Name: 16-001
• Applications: WB
• Recommended Dilution: ELISA.
  Western blot.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody rects with recombinant proteins-(His)6.
• Formulation: PBS pH7.4, with 15mM sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20 to -70 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
• Synonyms: 6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag