E Cadherin (CDH1) Mouse Monoclonal Antibody [Clone ID: 67A4]

CAT#: AM26015PU-N

E Cadherin (CDH1) mouse monoclonal antibody, clone 67A4, Aff - Purified

Specifications

Product Data

• Clone Name: 67A4
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow cytometry: Tested on cell lines CACO-2 and HT-29. In this case the recommended concentration is 5-10 μg/ml per 1 million cells/ml.
  Immunoprecipitation.
  Western blot.
  Immunohistochemistry (frozen sections): 4-8 μg/ml; positive tissue: tonsil.
  Immunocytochemistry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: T-47D cells
• Specificity: This antibody recognizes CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cadherin 1
• Database Link:
  Entrez Gene 999 Human
  UniProt ID P12830
• Background: CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.
• Synonyms: Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO