RP105 (CD180) Mouse Monoclonal Antibody [Clone ID: G28-8]

CAT#: AM26010PU-N

RP105 (CD180) mouse monoclonal antibody, clone G28-8, Aff - Purified

Specifications

Product Data

• Clone Name: G28-8
• Applications: FC, FN, IP
• Recommended Dilution: Flow cytometry: 2 μg/ml.
  Immunoprecipitation.
  Functional application: induction of B cell proliferation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human tonsillar B cells
• Specificity: This antibody detects CD180.
• Formulation: PBS with 15 mM sodium azide, approx. pH 7.4
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD180 molecule
• Database Link:
  Entrez Gene 4064 Human
  UniProt ID Q99467
• Background: CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
• Synonyms: LY64

Reference Data

• Protein Families: Druggable Genome, Transmembrane