DYKDDDDK Epitope Tag Mouse Monoclonal Antibody [Clone ID: G191]

CAT#: AM20711PU-N

DYKDDDDK Epitope Tag mouse monoclonal antibody, clone G191, Aff - Purified

Product Images

Western blot analysis of 293 cells transfected with D-tagged victors at different concentrations of the anti-D antibody. Lane 1: Highest concentration (0.5 mg/ml). Lane 4: Lowest concenttaion at (0.1 g/ml).

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  Western blot analysis of extract from 293 cells transfected with (Lane 1) or without (Lane 2) Flag-tagged Bad using Flag-Tag monoclonal Antibody Cat.-No AM20711PU-N/PU-S

Specifications

Product Data

• Clone Name: G191
• Applications: IF, IP, WB
• Recommended Dilution: Western Blot: 1/1000-1/4000.
  Immunoprecipitation : 1/200.
  Immunofluorescence : 1/100.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH.
• Specificity: This antibody Recognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
• Formulation: PBS (pH 7.2) with 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
• Synonyms: FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag