ISG15 (1-157) Mouse Monoclonal Antibody [Clone ID: 3E5]

CAT#: AM09021PU-S

ISG15 (1-157) mouse monoclonal antibody, clone 3E5, Purified

Product Images

Cell lysates of HeLa (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

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  Western blot analysis: Cell lysates of HeLa (each 50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 3E5
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:1,000 - 1:2,000).
  Immunocytochemistry / Immunoflourescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human ISG15 (1-157 aa) purified from E. coli
• Specificity: The antibody recognizes human ISG15.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ISG15 ubiquitin-like modifier
• Database Link:
  Entrez Gene 9636 Human
  UniProt ID P05161
• Background: ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.
• Synonyms: UCRP