Nuclei (Nuclear Marker) Mouse Monoclonal Antibody [Clone ID: 235-1]

CAT#: AM08257PU-S

Nuclei (Nuclear Marker) mouse monoclonal antibody, clone 235-1, Purified

Specifications

Product Data

• Clone Name: 235-1
• Applications: FC, IF, IHC, IP
• Recommended Dilution: Flow Cytometry: 1-2ug/million cells
  Immunofluorescence: 1-2ug/ml
  Immunoprecipitation: 1-2ug/500ug protein lysate
  Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT
  Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT
  Optimal dilution for any specific application should be determined.
  Recommended Positive Control: Tonsil
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Nuclei of human myeloid leukemia biopsy cells.
• Specificity: This Monoclonal Antibody is part of a new panel of rea gents, which recognizes subcellular organelles or compartments of Human cells.
  These marker s may be useful in identification of these organelles in cells, tissues, and biochemica l preparations. Monoclonal Antibody 235-1 recognizes an antigen associated with the nuclei in Human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear ma rker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
  Cellular Localization: Nuclei.
  Negative Species: Mouse, Rat, Chicken.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 70 kda and 80 kDa
• Background: Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
• Synonyms: Human Nuclear Antigen