LAMP1 Mouse Monoclonal Antibody [Clone ID: H4A3]

CAT#: AM08169PU-N

LAMP1 mouse monoclonal antibody, clone H4A3, Purified

Product Images

Western Blot analysis using CD107a / LAMP1 Antibody Cat.-No AM08169PU-N (Clone H4A3) at 2 ug/ml in 1 mg/ml lysate, HeLa 10 ug (Lane 1+2), Raw 20 ug (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti-Mouse IgG (H+L)-HRP as secondary antibody.

Specifications

Product Data

• Clone Name: H4A3
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot: 2 µg/ml.
  Flow Cytometry: ≤ 1 µg/10⁶ cells.
  Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes Human CD107a/LAMP-1. Other species not tested.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2 without preservatives.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: lysosomal associated membrane protein 1
• Database Link:
  Entrez Gene 3916 Human
  UniProt ID P11279
• Background: CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets, tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium. (Ref.1-7)
• Synonyms: LAMP-1, LAMPA, LGP120