Cd28 Hamster Monoclonal Antibody [Clone ID: PV-1]

CAT#: AM08038FC-N

Cd28 hamster monoclonal antibody, clone PV-1, FITC

Product Images

Immunofluoscent Staining: A 50/50 mixture of the A20 B-cell lymphoma and the EL-4 T-cell lymphoma was stained with Hamster anti-Mouse CD28-FITC. Large cells were gated and analyzed by flow cytometry. Amount Used: 3 ug/10e6 cells.

Specifications

Product Data

• Clone Name: PV-1
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 3 µg/10e6 cells. (Ref.8-10)
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Specificity: This antibody is specific to Murine CD28.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD28 antigen
• Database Link:
  Entrez Gene 12487 Mouse
  UniProt ID P31041
• Background: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4)
  CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. (Ref.5) CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.6-9)
• Synonyms: TP44