GRK2 Mouse Monoclonal Antibody [Clone ID: 3F8]

CAT#: AM06612SU-N

GRK2 mouse monoclonal antibody, clone 3F8, Ascites

Product Images

Western blot analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.

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  Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

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  Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.

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  Immunofluorescence analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 3F8
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western Bloting: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/10000.
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human GRK2 expressed in E. Coli.
  
• Specificity: This antibody reacts to GRK2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 80 kDa
• Gene Name: G protein-coupled receptor kinase 2
• Database Link:
  Entrez Gene 156 Human
  UniProt ID P25098
• Background: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. (provided by RefSeq)Tissue specificity: Expressed in peripheral blood leukocytes
• Synonyms: BARK, BARK1, GRK2, Beta-adrenergic receptor kinase 1