CRTC3 Mouse Monoclonal Antibody [Clone ID: 5G9]

CAT#: AM06581SU-N

CRTC3 mouse monoclonal antibody, clone 5G9, Ascites

Product Images

Western blot analysis using CRTC3 mouse mAb against Hela (1), Jurkat (2), Cos7 (3) and MCF-7 (4) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded breast cancer (left) and ovarian cancer (right) using CRTC3 mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded lung cancer (left) and esophagus cancer (right) using CRTC3 mouse mAb with DAB staining.

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  Immunofluorescence analysis of NTERA-2 cells using CRTC3 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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Specifications

Product Data

• Clone Name: 5G9
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human CRTC3 expressed in E. Coli.
• Specificity: Recognizes CRTC3
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 68 kDa
• Gene Name: CREB regulated transcription coactivator 3
• Database Link:
  Entrez Gene 64784 Human
  UniProt ID Q6UUV7
• Background: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR) .Tissue specificity: Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
• Synonyms: TORC3, TORC-3