MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: 5H4]

CAT#: AM06580SU-N

MEK4 (MAP2K4) mouse monoclonal antibody, clone 5H4, Ascites

Product Images

Western blot analysis using MAP2K4 antibody Cat.-No AM06580SU-N against HepG2 (1), K562 (2), and HEK293 (3) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K4 antibody Cat.-No AM06580SU-N with DAB staining.

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  Flow cytometric analysis of K562 cells using MAP2K4 antibody Cat.-No AM06580SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 5H4
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human MAP2K4 expressed in E. Coli.
• Specificity: Recognizes MAP2K4
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 42 kDa
• Gene Name: mitogen-activated protein kinase kinase 4
• Database Link:
  Entrez Gene 6416 Human
  UniProt ID P45985
• Background: This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.Tissue specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues .
• Synonyms: JNK-activating kinase 1, MAP Kinase Kinase 4, SAPK/ERK kinase 1, PRKMK4, SERK1, MEK-4, MAPKK4, MKK4