Aurora A (AURKA) Mouse Monoclonal Antibody [Clone ID: 4G10]

CAT#: AM06573SU-N

Aurora A (AURKA) mouse monoclonal antibody, clone 4G10, Ascites

Product Images

Western blot analysis using AURKA antibody Cat.-No AM06573SU-N against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) using AURKA antibody Cat.-No AM06573SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 4G10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human AURKA expressed in E. Coli.
• Specificity: Recognizes Aurora kinase A
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 48 kDa
• Gene Name: aurora kinase A
• Database Link:
  Entrez Gene 6790 Human
  UniProt ID O14965
• Background: Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
• Synonyms: AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2