CDC27 Mouse Monoclonal Antibody [Clone ID: 5C12]

CAT#: AM06470SU-N

CDC27 mouse monoclonal antibody, clone 5C12, Ascites

Product Images

Western blot analysis using CDC27 antibody Cat.-No AM06470SU-N against CDC27 (AA: 724-830)-hIgGFc transfected HEK293 cell.

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  Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 antibody Cat.-No AM06470SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 5C12
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CDC27 expressed in E. Coli.
• Specificity: Recognizes CDC27
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 91 kDa
• Gene Name: cell division cycle 27
• Database Link:
  Entrez Gene 996 Human
  UniProt ID P30260
• Background: Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
• Synonyms: CDC27Hs, H-NUC, D0S1430E, D17S978E