B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: 1H12]
Other products for "BRAF"
Specifications
Product Data | |
Clone Name | 1H12 |
Applications | ELISA, IF, IHC, WB |
Recommended Dilution | ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000. |
Reactivities | Human, Mouse |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Purified recombinant fragment of human BRAF expressed in E. Coli. |
Specificity | Recognizes Threonine Protein Kinase BRAF |
Formulation | State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide. |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | 87 kDa |
Gene Name | B-Raf proto-oncogene, serine/threonine kinase |
Database Link | |
Background | BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109 is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers. |
Synonyms | BRAF, BRAF1, RAFB1, p94 |
Reference Data |
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