Ryanodine Receptor (RYR1) Mouse Monoclonal Antibody [Clone ID: G-1]
CAT#: AM05906PU-N
Ryanodine Receptor (RYR1) mouse monoclonal antibody, clone G-1, Purified
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Specifications
Product Data | |
Clone Name | G-1 |
Applications | ELISA, FC, IF, IHC, WB |
Recommended Dilution | ELISA: 1/1000. Flow Cytometry: 1/500. Western blot: 1/100. Immunofluorescence: 1/100. Use Indirect Immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, not enzymatic, detection is required. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended. Immunohistochemistry on Frozen Sections: 1/100. |
Reactivities | Bovine, Canine, Human, Mouse, Porcine, Rat |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Short synthetic polypeptide corresponding to the C-terminal domain of the Ryanodine Receptor |
Specificity | This antibody reacts with the C-terminus cytoplasmic domain of Ryanodine Receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. This antibody reacts with Ryanodine Receptor (~500kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues). |
Formulation | PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | ryanodine receptor 1 |
Database Link | |
Synonyms | CCO; MHS; MHS1; RYDR; RYR; RYR-1; SKRR |
Reference Data | |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Calcium signaling pathway, Long-term depression |
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