SMC2 Mouse Monoclonal Antibody [Clone ID: E1M]

CAT#: AM05324PU-N

SMC2 mouse monoclonal antibody, clone E1M, Purified

Product Images

Western blot analysis: CAPE antibody staining of HeLa cell lysate.

Specifications

Product Data

• Clone Name: E1M
• Applications: WB
• Recommended Dilution: Western Blot: 1-5 µg/ml.
  Positive Control/Tissue Expression: HeLa cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 523-768 of Human CAP-E and Mouse myeloma cells.
• Specificity: Recognizes SMC2/CAPE.
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: The antibody can be shipped at ambient temperature.
  Store (in aliquots) at -20°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: structural maintenance of chromosomes 2
• Database Link:
  Entrez Gene 10592 Human
  UniProt ID O95347
• Background: CAP-E and CAP-C (Chromosome Associated Protein-E & C) are also SMC (Structural Maintenance of Chromosome) family members that form a heterodimeric complex required for mitotic chromosome condensation to achieve proper segregation of genetic information during subsequent cell division. hCAP-C/hCAP-E is a component of a multiprotein complex called condensin that interacts with phosphorylation of Histone H3 resulting in the mitotic chromosome condensation. The distribution patterns of the two heterodimeric complexes in interphase nucleus indicate independent behaviour of the two complexes during cell cycle. These results suggest that the two distinct complexes are involved in different aspects of mitotic chromosome organization in human cells.
• Synonyms: SMC protein 2, SMC-2, SMC2L1, CAP-E, Chromosome-associated protein E, XCAP-E homolog