CDK7 Mouse Monoclonal Antibody [Clone ID: MO1]

CAT#: AM05225PU-N

CDK7 mouse monoclonal antibody, clone MO1, Purified

Specifications

Product Data

• Clone Name: MO1
• Applications: IHC, IP, WB
• Recommended Dilution: Western Blot (1-10 µg/ml).
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Recommended positive control tissue: HeLa cells fixed with acetone/methanol.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant 221 amino acid fragment of the C terminus of p40mo15 recognizing a 40 kD protein.
• Specificity: This Cdk7 antibody, clone MO-1 recognizes Human Cyclin-dependent kinase 7.
• Formulation: PBS
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction.
  Preservative: 0.08% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A/G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cyclin-dependent kinase 7
• Database Link:
  Entrez Gene 1022 Human
  UniProt ID P50613
• Background: It plays a crucial role in the activation of cdk’s.
  Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
• Synonyms: CAK1, P39 Mo15, STK1, CDK-activating kinase