Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: AX.14]

CAT#: AM05205FC-N

Integrin alpha E (ITGAE) mouse monoclonal antibody, clone AX.14, FITC

Specifications

Product Data

• Clone Name: AX.14
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Monitoring of Mucosa-associated T Lymphocytes.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody reacts with Intestinal Lymphocytes (HML-1).
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: One year from despatch.
• Gene Name: integrin subunit alpha E
• Database Link:
  Entrez Gene 3682 Human
  UniProt ID P38570
• Background: The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood ymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in Peripheral blood and lymphoid organs.
• Synonyms: Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE
• Note: Protocol: PBMC:
  Add 10 µl of MAB/10e6 PBMC in 100 µl PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 µl of MAB/100 µl of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.