VCP Mouse Monoclonal Antibody [Clone ID: Hs-14]

CAT#: AM03029PU-N

VCP mouse monoclonal antibody, clone Hs-14, Purified

Product Images

Immunofluorescence analysis of VCP in acetone-permeabilized human sperms using monoclonal antibody Hs-14 demonstrates its location to the acrosome. (Normal spermiogram shown).

Specifications

Product Data

• Clone Name: Hs-14
• Applications: FC, IF, WB
• Recommended Dilution: Flow cytometry.
  Western blot.
  Immunocytochemistry: 10 μg/ml; Staining technique: Membrane permeabilization (acetone) is essential. Application note: The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Specificity: The antibody reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
• Formulation: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Precipitation methods and size-exclusion chromatography; purity > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: valosin containing protein
• Database Link:
  Entrez Gene 7415 Human
  UniProt ID P55072
• Background: VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
• Synonyms: 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein