Leptin (LEP) Mouse Monoclonal Antibody

CAT#: AM01365PU-N

Leptin (LEP) mouse monoclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant human Leptin when used with Biotin conjugated anti-Human Leptin antibody (cat. PP1041B) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
  Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, under non-reducing conditions.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly pure (>98%) recombinant Human Leptin
• Specificity: This antibody detects Leptin.
• Formulation: PBS without preservatives
  State: Azide Free
  State: Lyophilized (sterile filtered) purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: The lyophilized antibody is stable at room temperature for one month or at -20°C for longer. Following reconstitution it is stable at 2-8°C for six weeks.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: leptin
• Database Link:
  Entrez Gene 3952 Human
  UniProt ID P41159
• Background: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
  Defects in the Leptin gene are the cause of profound obesity and type II diabetes.
• Synonyms: LEP, OB, OBS, Obesity factor, Obese protein