AKT1 pSer473 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11E6]

CAT#: AM00106BT-N

AKT1 pSer473 (incl. pos. control) mouse monoclonal antibody, clone 11E6, Biotin

Specifications

Product Data

• Clone Name: 11E6
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 µg/ml.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH
• Specificity: This antibody specifically recognizes PKB phosphorylated at serine 473 in Western blot and ELISA applications.
• Formulation: PBS/0.09% Na-Azide/PEG and Sucrose
  Label: Biotin
  State: Liquid
• Concentration: lot specific
• Purification: Size exclusion chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  Entrez Gene 207 Human
  UniProt ID P31749
• Background: The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. Activated PKB is phosphorylated at serine 473 (mTOR/Rictor or DNA-PK) and threonine 308 (PDK1).
• Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT