GSK3 beta (GSK3B) pSer9 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 3A8]

CAT#: AM00067PU-N

GSK3 beta (GSK3B) pSer9 (incl. pos. control) mouse monoclonal antibody, clone 3A8, Purified

Product Images

Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK-3A8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

Specifications

Product Data

• Clone Name: 3A8
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0,05 µg/ml.
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
• Specificity: This antibody specifically recognizes GSK3beta phosphorylated at Ser 9 at 46 kDa.
• Formulation: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  Entrez Gene 2932 Human
  UniProt ID P49841
• Background: Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.
• Synonyms: GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B