pGFP-C-shLenti shRNA Vector

CAT#: TR30023

HuSH shRNA GFP Lenti Cloning Vector (pGFP-C-shLenti Vector), 5 ug

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Specifications

Product Data

• Control Type: Negative
• Fluorescent Label: GFP
• Viral Packaging: Lentiviral
• Feature:
  • pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
  • U6_F Primer can be used to sequence the target sequence after cloning.
• Download Vector Sequence:
• Components: 1 vial of 5 ug lyophilized pGFP-C-shLenti shRNA cloning vector
• Quality Control: This vector was sequence verified and functionally validated.
• Storage: Store the vector DNA at -20°C.
• Stability: Vector DNA is stable for at least one year from date of receipt when stored at -20°C.