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CAS9 Purified Streptococcus pyogenes Protein

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Cat. No. Description Datasheet Price Availability*  
TP790148 Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli, 500 pmol (Larger pack size?) $105 In Stock
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OriGene Data
Species:Streptococcus pyogenes Expression Host:E.coli
Expression cDNA Clone or AA Sequence
1
Tag:N-terminal HIS Predicted MW:165.0 kDa
Purity:> 95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:20 uM
Buffer:10mM Tris,300mM NaCl,0.1mM EDTA,1mM DTT, pH7.4, 50% glycerol.
Endotoxin: < 0.5 EU per 1 µg of the protein by the LAL
BioActivity: In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease (Cat# TP790148). The input DNA is XmnI-linearized vector (PS100074) was cleaved by with a synthetic sgRNA.
Protein Families:
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Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: RefSeq Size: RefSeq ORF:
LocusID: Cytogenetic:  
Synonyms :
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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